KMID : 0380620190510010018
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Korean Journal of Food Science and Technology 2019 Volume.51 No. 1 p.18 ~ p.23
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Saccharification and alcohol fermentation characteristics of a mixture of tapioca and hulled barley
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Kim Sun-Hye
Oh Jong-Soo Kang Sung-Tae
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Abstract
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An enzymatically saccharified tapioca and hulled barley (TB) raw mixed solution was used to examine alcohol fermentation characteristics. The TB mixture was liquefied with 0.04% ¥á-amylase ¡°Spezyme-Fred¡± and saccharified using an enzyme mixture (GPB), which consisted of glucoamylase (G), protease (P), and ¥â-glucanase (B). After the TB mixture (7:3, w/w) saccharified for 150 min at 50oC, its glucose content was 12.9% and viscosity was 26 cp. The use of GPB for the saccharification of TB was appropriate because the addition of ¥â-glucanase increases the glucose yield and decreases the viscosity of the saccharification liquid. The TB ratio was optimized to 7:3 (w/w) on the basis of the lower viscosity and the higher glucose content after saccharification. After TB mixture with 300% (w/w) water content was better condition than others for alcohol fermentation when it was carried out at 30oC. The alcohol and glucose contents of the TB mixture fermented for 72 h were 9.0 and 0.02%, respectively, and the pH and total acidity were 4.3 and 0.3%, respectively.
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KEYWORD
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tapioca, hulled barley, alcohol fermentation, saccharification
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